Molecular characterization of zinc finger cDNA in pili (Canarium ovatum Engl.) pulp

Date

4-2014

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Roberta N. Garcia

Abstract

One of the most significant events in the expression and functioning of proteins is transcription--through the action of transcription factors aided by DNA-binding motifs such as zinc fingers, the most abundant in eukaryotes. Total RNA was extracted from one of the most underutilized crops in the world, pili (Canarium ovatum Engl.) specifically of Katutubo variety. Using Rapid Amplification of cDNA Ends (RACE) system of amplification, a novel 5?-RACE product of the upstream region of the gene was characterized, sequenced and was overlapped to the previously cloned and characterized 3?-RACE sequence, resulting to a 792-bp full-length nucleotide sequence encoding a 263- amino acid zinc finger (ZnF) protein of pili pulp, which was identified to be of PHD-finger type based on the nucleotide and protein multiple sequence analyses. The position of the motif was determined and was designated as a zinc finger PHD-type signature in association with the gene?s function on DNA binding, chromatin remodeling, and transcriptional regulation.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2014 A127 R58

Document Type

Thesis

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