Genotype analysis of roundabout, axon guidance receptor, homolog 2 gene in Philippine native chicken (Gallus gallus domesticus L.)

Date

6-2016

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Medino Gedeun N. Yebron Jr.

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

A total of 115 native chickens in the Philippines composed of seven genetic groups (Parawakan, Boholano, Labuyo, Darag, Patani and Banaba) and 28 commercial chickens (broilers and layers) were genotyped for Robo2 to determine their association with disease resistance against Newcastle disease. Genotype analysis for the resistant T allele of the Robo2 gene was done using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). Results revealed 65 TT genotype (resistant), 27 TC genotype and 23 susceptible (CC genotype) in native chickens. All seven (7) genetic groups have generally higher frequency of resistant T allele than the susceptible C allele. Parawakan is the most significantly associated genetic group with disease resistance, followed by Joloanon while the least associated are Darag and Banaba (X²= 17.90). The genetic groups of native chickens are significantly associated with disease resistance than commercial chicken (X²= 5.36). The black plumage color is also significantly associated with disease resistance (X²= 7.65). These results show the significant association of the genetic groups of native chickens and resistance to Newcastle disease that may be used for future breeding programs for Newcastle disease resistance.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2016 A127 /M66

Notes

Major in Animal Biotechnology

Document Type

Thesis

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