Serological and molecular detection of Newcastle disease virus from raptors in Biodiversity Management Bureau, Quezon City, Philippines

Date

6-2016

Degree

Doctor of Veterinary Medicine

College

College of Veterinary Medicine (CVM)

Adviser/Committee Chair

Dennis V. Mali

Abstract

Newcastle disease (ND) is a highly contagious and often severe disease found worldwide that affects birds including domestic poultry and wild birds. Wild birds tend to carry the disease asymptomatically, rapidly spreading the disease in the wild. This study can provide important information regarding the local epidemiology of NDV in raptors in the Philippines, and could help identify potential threats of wild birds, in the spread of NDV in domestic poultry. Serological and molecular detection of NDV were performed in apparently healthy and clinically ill raptors in a wildlife rescue centre in Quezon City. Sera were tested for antibodies against Newcastle Disease using hemagglutination inhibition test and results showed that out of 42 birds, 16 birds (38.11%) had varying levels of antibody titers against NDV. The NDV antibody titers had a range of 1:128 (27) to 1:1024 (210) with a geometric mean titer of 29. Molecular confirmation using nested RT-PCR using tracheal and cloacal swabs showed that all samples were negative for NDV. This suggests that there is no ongoing infection of NDV on the birds sampled, but exposure to the disease prior to capture occurred.

Language

English

Location

UPLB College of Veterinary Medicine (CVM)

Call Number

LG 993.5 2016 V4 T9

Document Type

Thesis

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