Callus induction in cotyledons of Moringa oleifera lam

Issue Date

9-2011

Abstract

Callus cultures of Moringa oleifera Lam. were successfully established from cotyledon explants obtained from green mature seeds. Benzyladenine (BA) alone (2.5 mg L -1) significantly increased callus formation but was not significantly different when combined with increasing levels of 2,4-dichlorophenoxyacetic acid (2,4-D) (1.0-5.0 mg L -1) and further supplemented with thidiazuron (TDZ). Loose crystalline calli were observed in media containing 2,4-D and BA singly or in combination, while compact, nodular and loose calli were obtained when the culture media were further enriched with TDZ. BA and 2,4-D singly or in combination did not significantly enhance degree of callus formation but BA in combination with 1.0 and 5.0 mg L -1 2,4-D and TDZ significantly increased degree of callusing. A significant increase in callus weight was observed in BA at different levels combined with 2,4-D (1.0 and 2.5 mg L -1). Addition of TDZ to BA and 2,4-D containing media further increased callus weight. Slight greening of calli was observed 4 wk after the first transfer onto the shoot induction media; however, statistical analysis revealed that there was no significant difference among treatments. Microscopic observation of hand-sectioned protrusions on green calli revealed heterogenous and asynchronous cell growth showing actively dividing cells. Xylem differentiation was also evident, indicating initiation of bud initials.

Source or Periodical Title

Philippine Agricultural Scientist

ISSN

0031-7454

Volume

94

Issue

3

Page

239-247

Document Type

Article

Language

English

Subject

24-D, BA, Callus, In-vitro, Moringa oleifera Lam, TDZ, Xylem differentiation

Digital Copy

yes

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