Genetic characterization of brassica rapa chinensis l., b. rapa parachinensis (L. H. Bailey) hanelt, and b. oleracea alboglabra (L. H. Bailey) hanelt using simple sequence repeat markers

Issue Date

12-2009

Abstract

Genetic diversity of 39 accessions of Brassica rapa chinensis L., 28 accessions of B. rapa parachinensis (L. H. Bailey) Hanelt, and 29 accessions of B. oleracea alboglabra (L. H. Bailey) Hanelt was studied. Fifty-four SSR primers were used and produced 122 scorable bands in which 77 were polymorphic. The average rate of polymorphic loci was 71.08% which indicates high genetic diversity among the accessions. Phylogenetic analysis showed that B. rapa chinensis and B. rapa parachinensis are genetically closely related to each other. B. oleracea alboglabra accessions grouped together in a separate cluster. Groupings also reflected geographical similarities and may suggest misidentification of certain accessions in the germplasm collection. Thus, SSR analysis proved to be a useful tool in assessing the genetic diversity of leafy Brassica germplasm.

Source or Periodical Title

Philippine Journal of Science

ISSN

317683

Volume

138

Issue

2

Page

141-152

Document Type

Article

College

College of Arts and Sciences (CAS)

Language

English

Subject

Brassica oleracea, Brassica rapa, Dendrogram, Genetic diversity, Geographical origin, Microsatellites, Simple sequence repeats

Digital Copy

None

Link to Full Text

Share

COinS