Rapid evaluation protocol for hydrogen peroxide and ultraviolet radiation resistance using lesion mimic mutant rice (Oryza sativa L.)

Issue Date

4-2011

Abstract

Ultraviolet (UV) radiation can induce an increase in hydrogen peroxide (H₂O₂) production which could both inflict injury to crops affecting growth nd productivity. Responses of plants to UV and H₂O₂ may vary not only among species but also among cultivars. A protocol for screening UV and H₂O₂ resistance was developed using lesion mimic mutant (LMM) rice lines to detect resistant rice breeding lines related to abiotic stresses where H₂O₂ is involved. To date, there is no standard method or protocol yet that has been defined for the rapid and reliable detection of resistant rice lines using UV and H₂O₂. Screening under field conditions is difficult due to plant and soil interactions, soil heterogeneity, land and labor resources, and uncontrollable environmental effects such as temperature, humidity, rainfall, and solar radiation/duration. Thus, a rapid and reproducible laboratory protocol for H₂O₂ and UV radiation is necessary to reduce time and resources with desirable outputs. LMM rice lines were preferably used than the cultivars since LMM are known to be more sensitive to stress signals and spontaneously develop necrotic lesions when subjected to environmental stresses. Rice seedlings were subjected to 50, 100, 200, 400, 600, and 1000 mM H₂O₂ concentration for 3 d. This resulted to different reaction of leaf browning and 600 mM H₂O₂ concentration was selected to screen leaf and plant samples. For UV treatment, seedlings were exposed to artificial UV light at 30 cm height. Results showed that 3 out of 13 fully characterized lesion mimic mutant lines had 50% survival under ultraviolet stress and 10 out of 52 other lesion mimic mutants were found to be tolerant to H₂O₂ better than the wild type Dongjin. Our investigation indicated that H₂O₂ could be used as selectable marker in the early detection of resistant lines to related abiotic stresses where H₂O₂ has been involved. H₂O₂ is known as a regulatory molecule in stress perception and signal transduction.

Source or Periodical Title

Philippine Journal of Crop Science

ISSN

0115-463x

Volume

36

Issue

1

Page

19-27

Document Type

Article

Frequency

tri-quarterly

Physical Description

tables, graphs, picture

Language

English

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