Sub-acute Oral Toxicity Evaluation of Philippine Bignay (Antidesma bunius (L.) Spreng cv. ‘Kalabaw’) Fruit in ICR Mice

Authors

Jonina Marie Tengco, Institute of Human Nutrition and Food, College of Human Ecology, University of the Philippines Los Baños (UPLB), Laguna 4031, Philippines
Liezl Atienza, Institute of Human Nutrition and Food, College of Human Ecology, University of the Philippines Los Baños (UPLB), Laguna 4031, PhilippinesFollow
Maria Amelita Estacio, Department of Basic Veterinary Sciences, College of Veterinary Medicine, UPLB, Laguna 4031, Philippines
Mark Joseph Desamero, Department of Basic Veterinary Sciences, College of Veterinary Medicine, UPLB, Laguna 4031, Philippines
Roxanne Gapasin, Department of Basic Veterinary Sciences, College of Veterinary Medicine, UPLB, Laguna 4031, Philippines
Jonna Rose Maniwang, Department of Basic Veterinary Sciences, College of Veterinary Medicine, UPLB, Laguna 4031, Philippines
Katherine Ann Castillo-Israel, Institute of Food Science and Technology, College of Agriculture and Food Science, UPLB, Laguna 4031, Philippines
Rohani Navarro, National Institute of Health, University of the Philippines Manila, Manila 1000, Philippines
Dianne Jane Sunico, Institute of Human Nutrition and Food, College of Human Ecology, University of the Philippines Los Baños (UPLB), Laguna 4031, Philippines
James Ryan Aranzado, Institute of Human Nutrition and Food, College of Human Ecology, University of the Philippines Los Baños (UPLB), Laguna 4031, Philippines
Ann Cayetano, Institute of Human Nutrition and Food, College of Human Ecology, University of the Philippines Los Baños (UPLB), Laguna 4031, Philippines
Joan Delomen, Institute of Human Nutrition and Food, College of Human Ecology, University of the Philippines Los Baños (UPLB), Laguna 4031, Philippines
Loraine Ancheta, Institute of Food Science and Technology, College of Agriculture and Food Science, UPLB, Laguna 4031, Philippines

Publication Date

12-1-2022

Abstract

This study evaluated the safety of Bignay (Antidesma bunius (L.) Spreng cv. ‘Kalabaw’) fruits through a sub-acute oral toxicity test in male and female ICR mice. The evaluation was done through oral gavage of the aqueous fruit sample at 2,000 mg kg^-1 body weight dose for 28 d. Results showed that there were no significant changes in feed and water intake of male and female ICR mice as compared to the control groups that received distilled water (p < 0.05). Blood chemistry and hematology values for all mice groups were comparable to the controls and published values. All mice groups also had appreciable body weight gain (10.89% – 21.69%) with zero morbidity and mortality. Lastly, the gross and microscopic examination of the brain, heart, lungs, GI tract, liver, spleen, and kidneys showed normal architecture and histology, suggesting that the fruits did not induce any morphological abnormalities. Results of this study showed that Bignay (cv. ‘Kalabaw’) fruits are safe for consumption with an LD₅₀ of greater than 2,000 mg kg^-1 body weight.

Recommended Citation

Tengco, Jonina Marie; Atienza, Liezl; Estacio, Maria Amelita; Desamero, Mark Joseph; Gapasin, Roxanne; Maniwang, Jonna Rose; Castillo-Israel, Katherine Ann; Navarro, Rohani; Sunico, Dianne Jane; Aranzado, James Ryan; Cayetano, Ann; Delomen, Joan; and Ancheta, Loraine (2022) "Sub-acute Oral Toxicity Evaluation of Philippine Bignay (Antidesma bunius (L.) Spreng cv. ‘Kalabaw’) Fruit in ICR Mice," The Philippine Agricultural Scientist: Vol. 105: No. 4, Article 5.
Available at: https://www.ukdr.uplb.edu.ph/pas/vol105/iss4/5