The Philippine Agricultural Scientist
Publication Date
12-1-2022
Abstract
Grammatophyllum multiflorum var. ‘tigrinum’ Lindley is an endangered orchid species endemic to the Philippines. To ensure its sustainable utilization as a source of phytochemicals, the types and concentrations of cytokinin, and auxin for shoot and root induction using nodal explants from in vitro germinated seedlings for its mass propagation were established. Seeds were germinated in vitro using MS medium without growth regulator. The nodal explants from the in vitro germinated seedlings were placed in MS medium containing different cytokinins and auxins to optimize multiple shoots and root formation, respectively. Thidiazuron (TDZ) at 2.5 μM was found to be the best cytokinin for multiple shoot formation, while naphthaleneacetic acid (NAA) at 5 μM was found to be the best auxin for root formation. Rooted plantlets were potted out using coir as medium and hardened in the nursery under partial shade condition. No abnormality was observed in terms of growth and morphology between the mother plant and the tissue culture-derived plants. Antimicrobial bioassays of the extracts from different parts of the tissue culture-derived G. multiflorum showed remarkable antimicrobial activities suggesting the potential of this orchid as a source of important antimicrobial compounds.
Recommended Citation
Marfori, Eufrocinio
(2022)
"Micropropagation of Grammatophyllum multiflorum var. 'tigrinum' Lindley and Evaluation of its Antimicrobial Activities,"
The Philippine Agricultural Scientist: Vol. 105:
No.
4, Article 6.
Available at:
https://www.ukdr.uplb.edu.ph/pas/vol105/iss4/6