Effects of Culture Media and Duration on In Vitro Maturation and Production of Cỏ Goat Embryos

Authors

Van Khanh Nguyen, Key Laboratory of Animal Cell Biotechnology, National Institute of Animal Sciences, Hanoi, VietnamFollow
Huong Thi Thu Vu, Key Laboratory of Animal Cell Biotechnology, National Institute of Animal Sciences, Hanoi, Vietnam
Au Thi Hoang, Key Laboratory of Animal Cell Biotechnology, National Institute of Animal Sciences, Hanoi, Vietnam
Yen Thi Kim Pham, Key Laboratory of Animal Cell Biotechnology, National Institute of Animal Sciences, Hanoi, Vietnam
Dat Van Le, Key Laboratory of Animal Cell Biotechnology, National Institute of Animal Sciences, Hanoi, Vietnam
Lan Anh Thi Nguyen, Key Laboratory of Animal Cell Biotechnology, National Institute of Animal Sciences, Hanoi, Vietnam
Huu Xuan Quan, Key Laboratory of Animal Cell Biotechnology, National Institute of Animal Sciences, Hanoi, Vietnam
Lan Doan Pham, Key Laboratory of Animal Cell Biotechnology, National Institute of Animal Sciences, Hanoi, Vietnam

Publication Date

12-1-2024

Abstract

This study aimed to evaluate the effects of maturation media and duration on the in vitro maturation (IVM) of Cỏ goat oocytes for the in vitro production of Cỏ goat embryos. In Experiment 1, the highest percentage of matured oocytes and blastocyst rates were observed in the IVM medium consisting of tissue culture medium (TCM) 199 + 10% fetal calf serum (FCS) + 50 ng/mL follicle stimulating hormone (FSH) + 100 μM cysteamine + 10 ng/mL epidermal growth factor (EGF) + 100 units/mL penicillin G potassium + 0.1 mg/mL streptomycin sulphate (P < 0.05). In Experiment 2, Cỏ goat oocytes were cultured in the same IVM medium for 20, 22, and 24 h, respectively. The matured oocyte and blastocyst rates of the 20-h group were lower than those of the 22- and 24-h groups (61.56% vs. 76.81% and 73.48%; 13.76% vs. 24.75% and 24.72% respectively; P < 0.05), while there was no difference between the 22- and 24-h groups (P > 0.05). In conclusion, the TCM 199 medium supplemented with FCS, FSH, cysteamine, EGF, and a maturation duration of 22 or 24 h are suitable for the in vitro maturation and subsequent embryo development of Cỏ goat oocytes.

Recommended Citation

Nguyen, Van Khanh; Vu, Huong Thi Thu; Hoang, Au Thi; Pham, Yen Thi Kim; Le, Dat Van; Nguyen, Lan Anh Thi; Quan, Huu Xuan; and Pham, Lan Doan (2024) "Effects of Culture Media and Duration on In Vitro Maturation and Production of Cỏ Goat Embryos," The Philippine Agricultural Scientist: Vol. 107: No. 4, Article 11.
Available at: https://www.ukdr.uplb.edu.ph/pas/vol107/iss4/11