Quality assurance in hemorrhagic septicemia
Professorial Chair Lecture
Southern California Filipino Veterinary Medical Association Professorial chair
Place
Assembly Hall, CVM-IAS Commercial Building, UPLB
Date
6-14-2001
Abstract
Hemorrhagic septicemia (HS) is a fatal disease mainly of cattle and buffaloes and caused by the Gram negative bacteria Pasteurella multocida 13:2 in Asia and E:2 in Africa. It is a disease of economic importance because of the high mortalities it causes. In many areas in the Philippines, HS is endemic and a practical method of control is vaccination. Oil adjuvant HS vaccine was produced at BIOTECH, UPLB in cooperation with CVM-UPLB. The Pasteurella millrace& 13:2 seed culture GI, used was isolated from HS case in Northern Samar. The HS vaccine passed the quality control (QC) requirements of BAI for the following: During production: for colony characteristic of Pasteurella multocida on visual examination and for purity on Gram staining. Post-production on the final product: for sterility, safety. potency and shelf life. Results of the sterility test showed the vaccine to be free from bacterial. fungal and viral contaminants. Safety test results showed that all mice did not manifest adverse reaction to the vaccine except for the granuloma formation which was expected in the injection site. The vaccine was subdivided into Group IA. I B and IC and subjected to the different conditions at 4°C in the refrigerator. 24 hours inside refrigerator without electricity and at room temperature (28 - 30°C) for 24 hours, respectively: then were subjected to potency test. A 100% protection rate was obtained for all subgroups and there was no significant difference in their protection rate (P>0.05). Two and three months post-production, the same vaccine revealed a 100°4 protection rate similar to Group I subgroups and no significant differences were shown among the different groups (P>0.05). Our oil adjuvant HS vaccines passed the quality control requirements of BAI and we hope our future vaccines will likewise pass the tests. Presently. more field trials are being conducted on the vaccine.
Location
UPLB Main Library Special Collections Section
Call Number
PL
Recommended citation
Molina, Helen A., "Quality assurance in hemorrhagic septicemia" (2001). Professorial Chair Lecture. 102.
https://www.ukdr.uplb.edu.ph/professorial_lectures/102
Pages/Collation
20 leaves