Quality assurance in hemorrhagic septicemia

Professorial Chair Lecture

Southern California Filipino Veterinary Medical Association Professorial chair

Place

Assembly Hall, CVM-IAS Commercial Building, UPLB

Date

6-14-2001

Abstract

Hemorrhagic septicemia (HS) is a fatal disease mainly of cattle and buffaloes and caused by the Gram negative bacteria Pasteurella multocida 13:2 in Asia and E:2 in Africa. It is a disease of economic importance because of the high mortalities it causes. In many areas in the Philippines, HS is endemic and a practical method of control is vaccination. Oil adjuvant HS vaccine was produced at BIOTECH, UPLB in cooperation with CVM-UPLB. The Pasteurella millrace& 13:2 seed culture GI, used was isolated from HS case in Northern Samar. The HS vaccine passed the quality control (QC) requirements of BAI for the following: During production: for colony characteristic of Pasteurella multocida on visual examination and for purity on Gram staining. Post-production on the final product: for sterility, safety. potency and shelf life. Results of the sterility test showed the vaccine to be free from bacterial. fungal and viral contaminants. Safety test results showed that all mice did not manifest adverse reaction to the vaccine except for the granuloma formation which was expected in the injection site. The vaccine was subdivided into Group IA. I B and IC and subjected to the different conditions at 4°C in the refrigerator. 24 hours inside refrigerator without electricity and at room temperature (28 - 30°C) for 24 hours, respectively: then were subjected to potency test. A 100% protection rate was obtained for all subgroups and there was no significant difference in their protection rate (P>0.05). Two and three months post-production, the same vaccine revealed a 100°4 protection rate similar to Group I subgroups and no significant differences were shown among the different groups (P>0.05). Our oil adjuvant HS vaccines passed the quality control requirements of BAI and we hope our future vaccines will likewise pass the tests. Presently. more field trials are being conducted on the vaccine.

Location

UPLB Main Library Special Collections Section

Call Number

PL

Pages/Collation

20 leaves

This document is currently not available here.

Share

COinS